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. 2015 Aug 28;5(3):385–398. doi: 10.3390/membranes5030385

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Epifluorescence images showing patterns of two-bilayer SLB stacks consisting of a homogeneous bottom bilayer and a phase-segregated top bilayer. The first lipid bilayer (composed of DOPC: DOTAP: DHPE-OG) is observed in the green channel, while the second lipid bilayer (composed of DOPC: DSPC: DOPS: DHPE-LR) can be observed in the red channel. The formation of the second bilayer leads to significant fluorescence intensity changes in the first bilayer. The overlaid image of the two bilayers shows the fluorescence patterns are complimentary, due to FRET.