Figure 5.

Epifluorescence images of stacked phase-segregated SLB patterns. The first lipid bilayer was composed of DOPC:DSPC:DOTAP:DHPE-OG (green channel), while the second lipid bilayer was composed of DOPC:DSPC:DOPS:DHPE-LR (red channel). Insets show zoomed-in areas of the patterned bilayers. (a) When the first bilayer showed good phase segregation (green), the second lipid bilayer would phase segregate in the same pattern (red). The fluorescence intensity of first bilayer would decrease due to FRET, and the overlaid image of the two channels showed alignment of gel phase domains in the two bilayers; (b) When the first bilayer did not phase-segregate, the second SLB would still show gel-phase domains. The first bilayer appeared dimmer in areas that overlapped with the liquid disordered phase of the second bilayer. Image contrast has been adjusted to facilitate the visualization of the overlapping patterns in the stacked lipid bilayers.