Figure 4.

AdO suppressed the LPS-induced toll-like receptor 4 (TLR4) expression and nuclear factor (NF)-κB activation. TLR4 expression was evaluated using immunofluorescence analysis (A) and Western blot analysis (B); (C) NF-κB p65 expression was examined using immunofluorescence analysis; (D) The cytoplasm and nuclear proteins were extracted and the NF-κB p65 protein was analyzed by Western blot analysis. The immunofluorescence analysis was carried out by laser scanning confocal microscopy (60×), and the images were processed using ImageJ software. The immunofluorescence analysis and Western blot analysis were performed in three independent experiments.