Table 3.

Elution order of photosynthetic pigments from the diatom P. tricornutum and their visible absorption characteristics.

Pigment	Literature Data				HPLC Data *
	Solvent	λmax (nm)	E (L g−1·cm−1)	Reference	λmax (nm)	Retention Time (min)
Chlorophyll c1	acetone (90%)	443	318	[120]
Chlorophyll c2	acetone (90%)	444	374	[120]
Chlorophyll c1 + c2	N/A	N/A	N/A	N/A	443	4.7
Fucoxanthin	petrol ether	449	165	[121]	447	7.0
	ethanol	450	114	[122]
	acetone	443	165	[123]
Violaxanthin	N/A	443	N/A	[114]	441	8.4
Diadinoxanthin	acetone	448	224	[124]	447	9.9
	methanol	445	225	[124]
	hexane	446	211	[124]
Anteraxanthin	ethanol	446	235	[125]	447	10.8
Diatoxanthin	acetone	454	N/A	[114]	454	11.3
Zeaxanthin	ethanol	452	254	[126]	453	11.9
Chlorophyll a	acetone	662	88.15	[127]	429	14.9
β-carotene	etanol	453	262	[128]	454	18.7
	hexane	453	259.2	[128]
	acetone	454	250	[129]

*: data obtained with the method by Kraay andco-workers [117] for pigment extracts from the diatom P. tricornutum.