Table 2. Inter-Assay Reproducibility of HCV Real-Time RT-PCR.
Day | Input Copy Numbers of HCV 5’NCR RNA Control | ||||
---|---|---|---|---|---|
Threshold Cycle (Ct) | |||||
103 | 104 | 105 | 106 | 107 | |
1 | 29.21 | 26.47 | 22.06 | 19.88 | 15.28 |
2 | 29.50 | 26.61 | 23.30 | 19.23 | 15.91 |
3 | 30.10 | 26.03 | 21.54 | 19.06 | 15.12 |
4 | 29.43 | 25.40 | 22.21 | 19.41 | 14.79 |
5 | 28.65 | 27.23 | 20.87 | 20.37 | 16.32 |
Values a | 29.39 ± 0.52 | 26.34 ± 0.68 | 21.99 ± 0.89 | 19.61 ± 0.50 | 15.48 ± 0.61 |
CV b | 1.76 | 2.58 | 4.04 | 2.54 | 3.94 |
a Data are presented as mean ± SD.
b Abbreviation: coefficient of variation.