Table 3. Intra-Assay Accuracy of HCV Real-Time RT-PCR.
Tube | Input Copy Numbers of HCV 5’NCR RNA Control | ||||
---|---|---|---|---|---|
Threshold Cycle (Ct) | |||||
103 | 104 | 105 | 106 | 107 | |
1 | 28.70 | 26.15 | 21.22 | 19.76 | 15.30 |
2 | 29.12 | 26.28 | 21.05 | 19.98 | 15.06 |
3 | 28.54 | 25.83 | 21.46 | 19.49 | 15.53 |
4 | 28.61 | 26.38 | 21.90 | 19.51 | 15.74 |
Values a | 28.74 ± 0.26 | 26.40 ± 0.30 | 21.40 ± 0.36 | 19.68 ± 0.23 | 15.40 ± 0.29 |
CV b | 0.90 | 1.13 | 1.68 | 1.16 | 1.88 |
a Data are presented as mean ± SD.
b Abbreviation: coefficient of variation.