Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 28;12:150. doi: 10.1186/s12985-015-0383-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Palma-Ocampo et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — DENV-2 induces the expression of IFN-III in C33-A cells. C33-A cells were treated with IFN-λ1 (35 ng/ml) infected with DENV-2 (MOI = 0.1) or pre-treated with IFN-λ1 and subsequently infected with DENV-2. Forty-eight h post-infection, total cellular RNA was isolated and used in end-point RT-PCR for qualitative determination of the IFN-λ1, IFN-λ2, IFN-λ3 genes, using HPRT as an endogenous control gene. a Results of RT-PCR amplification of mock-infected and the three experimental conditions are shown. b Extracts of total protein from each condition were used for Western blotting to determine the presence of IFN-λ1 and IFN-λ2 proteins. Actin was the loading control. A semi quantitative analysis of detected bands normalized to actin was performed using ImageJ software (http://rsb.info.nih.gov/ij/)