Table 1.
Primers used in the present study
| Gene name and accession number in GenBank | Sequence (5′–3′) | UPL sequence (UPL#)a | Amplicon bp | Alignmentb |
|---|---|---|---|---|
| MX1 | TCCAGCCACCATTCCAAG | TTCTCCTG (2) | 60 | 868–885 |
| NM_001144925.2 | CAACAAGTTAAATGGTATCACAGAGC | 927–902 | ||
| OAS1 | GGTGGAGTTCGATGTGCTG | CCAGGGCA (37) | 64 | 538–556 |
| XM_006719434.1 | AGGTTTATAGCCGCCAGTCA | 601–582 | ||
| IFN-β | CTTTGCTATTTTCAGACAAGATTCA | CTGGCTGG (20) | 69 | 336–360 |
| NM_002176.2 | GCCAGGAGGTTCTCAAGAAT | 404–389 | ||
| IFN-λ1 | TCCTAGACCAGCCCCTTCA | c | 73 | 435–453 |
| NM_172140.1 | GTGGGCTGAGGCTGGATA | 507–490 | ||
| IFN-λ2 | CCTGGTGGACGTCTTGGA | c | 84 | 406–423 |
| NM_172138.1 | GTGGGCTGAGGCTGGATA | 489–472 | ||
| IFN-λ3 | AGGGCCAAAGATGCCTTAG | c | 123 | 167–185 |
| NM_172139.2 | CAGCTCAGCCTCCAAAGC | 298–281 | ||
| HPRT1 | – | – | 127 | – |
| NM_000194.2d |
aUPL: Universal Probe Library, Roche Life Science. Number of the probe is in parentheses
bPosition of primers in the corresponding gene sequences
cSybrGreen dye was used
dSpecific gene positions and sequences of Applied Biosystems primers and probes are not disclosed