Table 2.
IFN-γ responses following co-culture with the 9-11mer peptide library.
| SLA molecule | Pig | Adjuvant | Total number of responses | Stable binders | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 12 | Day 33 | Day 40 | Day 54 | Day 12 | Day 33 | Day 40 | Day 54 | |||
|
1*04:01 3*04:01 |
2034 | CASAC | 11 | 3 | 1 | 0 | 3(27.2%) | 1(33.3%) | 1(100.0%) | – |
| 2043 | ISA 51 VG | 2 | 0 | 0 | 0 | 0(0.0%) | – | – | – | |
| 1*07:02 | 2038 | CAF09 | 2 | 1 | 0 | 1 | 0(0.0%) | 0(0.0%) | – | 1(100.0%) |
| 2042 | CASAC | 31 | – | 6 | 6 | 9(29.0%) | – | 3(50.0%) | 3(50.0%) | |
| 2040 | PBS | – | 0 | – | 9 | – | – | – | 3(33.3%) | |
|
1*04:01 1*07:02 3*04:01 |
2035 | CAF09 | 4 | 2 | 0 | 3 | 1(25%) | 1(50.0%) | – | 2(66.6%) |
| 2046 | ISA 51 VG | 1 | 3 | 0 | 0 | 1(100.0%) | 1(33.3%) | – | – | |
|
1*04:01 2*05:02 3*04:01 |
2041 | ISA 51 VG | – | – | 2 | 6 | – | – | 0(0.0%) | 2(33.3%) |
Summarized responses from PBMCs co-cultured with the 9-11mer peptide library. Pigs are divided into four groups based on their SLA-profile. Adjuvant indicates immunization strategy, i.e., CAF09, CASAC, ISA 51 VG, or PBS injected animals. The total number of responses at day 12, 33, 40, and 54 are shown (column 4–7) as well as the number of responses generated from co-culture with a peptide shown to stably bind the given SLA molecule (column 8–11). The percentage of responses generated from these stable binders is shown in brackets for each time point. Animals with no responses at any time point are left out of the analysis. “–“ indicates that the quality control thresholds of the assay were not fulfilled and therefore these data were left out of the analyses.