Table 2.

Measurement of the by-product H₂O₂.

Cofactor	FAD [mM H2O2]	LpNox [mM H2O2]	FAD/LpNox [mM H2O2]
MNAH	0.32 ± 0.04	0.2*10-3± 0.1*10-3	16*10-3 ± 1*10-3
BNAH	0.51 ± 0.18	2.6*10-3 ± 0.9*10-3	25*10-3 ± 1*10-3

H₂O₂ concentrations in the reactions of FAD or LpNox with 1 mM of the biomimetics MNAH and BNAH were detected with DA-64 and horseradish peroxidase. Firstly either FAD or the enzyme was added to the cofactor. Secondly a combined approach was performed with addition of LpNox after 1 h. The standard deviation was calculated from three measurements.