Table 2.

GC-MS-SIM conditions for the determination of endogenous ABA, GA, and JA.

GC-MS-SIM conditions
Equipment	Hewlett-Packard 6890, 5973N Mass Selective Detector
Column	DB-1 capillary column (30 m × 0.25 mm, i.d. 0.25 μm film thickness)
Carrier gas	He (40 mL·min−1)
Source temperature	250°C
Oven conditions	ABA: 60°C (1 min) → 15°C·min−1 → 200°C (1 min) →            5°C·min−1 → 250°C (1 min) → 10°C·min−1 →            285°C (5 min)
	GA: 60°C (1 min) → 15°C·min−1 → 200°C (1 min) →           5°C·min−1 → 285°C (5 min)
	JA: 60°C (2 min) → 10°C·min−1 → 140°C (3 min) →           3°C·min−1 → 170°C → 15°C·min−1 → 285°C           (8 min)
Injector temperature	200°C
Ionizing voltage	70 eV