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HPLC operation conditions for the analysis of salicylic acid.
| HPLC | |||||
|---|---|---|---|---|---|
| Model | Shimadzu LC-10 | ||||
| Detector | RF-10 Axl (fluorescence detector) | ||||
| Wavelength | Excitation 305 nm, emission 365 nm | ||||
| Column | HP hypersil ODS (particle size 5 μm, pore size 120 Å) | ||||
| Solvent A | 100% MeOH | ||||
| Solvent B | 100% water in 0.5% acetic acid | ||||
| 5 min | 2.5 min | 4.5 min | 5 min | 3 min | |
| Gradient | A: 30% | A: 40% | A: 60% | A: 30% | A: 30% |
| B: 70% | B: 60% | B: 40% | B: 70% | B: 70% | |
| Flow rate | 1 mL·min−1 | ||||
