Table 1.

The PCR mix for one PCR reaction.

	1*mix (μl)
MilliQ	0.42
10 × Hotstar Taq PCR buffer	0.50
dNTP mix, 25 mM	0.04
Primermix forward (1 pmol/μl)	1.00
Primermix reverse (1 pmol/μl)	1.00
Hotstar Taq DNA polymerase (5 U/μl)	0.04
Bisulfite-treated DNA (5 ng/ul)/genomic DNA (5 ng/ul) or negative control (MilliQ)	2.00
Total volume	5