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. 2015 Sep 17;6:287. doi: 10.3389/fgene.2015.00287

Table 4.

ampliconPredict result for the 290 bp LEP amplicon.

Name Base mass Desc < 15.9 Da
CpG_Unit_2 2260.426 CpG_2 NA
CpG_Unit_3 2509.587 CpG_3 NA
CpG_Unit_4 4524.87 CpG_4 NA
CpG_Unit_5 2878.821 CpG_5 NA

This is the prediction given by the EpiTYPER.r script taken from the RSeqMeth package. Four out of 5 CpGs are measured in separate fragments. The mass of the fragments range from 2260 Da till 4525 Da and no other fragments are located with 15.9 Da, indicating that the measurement will not be influenced by the signal for another fragment. Also no warnings are given of possible overlaps between CpG units. Also no CpG unit has the same mass as another.