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. 2015 Sep 23;6:261. doi: 10.3389/fphys.2015.00261

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Copyright © 2015 Rettenberger, Schulze, Breer and Haid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative PCR (qPCR) analysis of mRNA for the receptor types GPR92, GPRC6A, and CaSR in the murine gastric antrum from normal fed mice. (A) The relative expression levels of the three receptor types in the antral mucosa from five different mice. In all cases the results indicate the highest expression rates for the peptone-receptor GPR92.(B) Mean transcript levels of GPR92, GPRC6A and CaSR from A1-A5 shown in (A). The values for GPR92 are 4.6-fold higher than those for GPRC6A (P = 0.004) and 8.1-fold higher than for CaSR (P = 0.0003). Ribosomal protein L8 (RpL8) was used as an internal control. Relative expression was calculated using the formula: ΔC_t = E^{Ct(target)−Ct(reference)} with corresponding efficiencies (E_GPR92: 1.988, E_GPRC6A: 1.967 and E_CaSR: 1.965). Data are generated in triplicate are expressed as mean ± SD with n = 5 mice. Statistically significant results determined by the unpaired t-test (^***P = 0.001).