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. 2015 Sep 23;6:997. doi: 10.3389/fmicb.2015.00997

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Copyright © 2015 Li, Niu, Li, Lei, Liu, Zhao, Guo, Zou, Yuan, Liu, Yuan and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity of the LAMP reaction and PCR for detection of the bla_OXA-51-like gene. Genomic DNA was diluted in a set of serial 10-fold dilutions. Both LAMP reactions (A,B) and PCR (C) were performed in duplicate for each dilution. Tubes and lanes: 1: 500 ng/μl, 2: 50 ng/μl, 3: 5 ng/μl, 4: 500 pg/μl, 5: 50 pg/μl, 6: 5 pg/μl, 7: 0.5 pg/μl, 8: 0.05 pg/μl. (A) Turbidity was monitored using a Loopamp real-time turbidimeter by measuring the absorbance at 650 nm every 6 s. (B) The direct visual method for the detection of LAMP. One microliter of fluorescent detection reagent was added to 25 μl of LAMP reaction mixture before the LAMP reactions were initiated. (C) PCR products were separated by 1% agarose gel electrophoresis and stained with ethidium bromide.