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. 2015 Sep 22;6:1024. doi: 10.3389/fmicb.2015.01024

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Copyright © 2015 Sánchez-Román, Puente-Sánchez, Parro and Amils.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TEM images of the bioprecipitates formed in T. Lapidicaptus anaerobic cultures. (A) The bioprecipitates are nanoparticles attached to T. lapidicaptus cell and its secreted EPS, respectively. (B) Electron diffraction pattern of the darker areas, more mineralized areas. (C) T. lapidicaptus cells with mineralized cell wall and covered by nanoparticles embeded in EPS. (D) Detail of T. lapidicaptus cell with mineralized cell wall. Note nanoparticles embedded in EPS. (E) Detail of three cells together with mineralized cell wall and EPS. The upper cell covered by nanoparticles embedded in EPS. (F) Elongated nanoparticle, vivianite nanocrystal, embedded in EPS. (G) Electron diffraction pattern of the nanocrystal (F). (H,I) EDX spectra of both dark and lighter mineralized areas (1A) composed of Fe-carbonate and phosphate, respectively. (J) EDX spectrum of nanoparticle (1E) composed of Fe-phosphate (vivianite).