FIGURE 3.

Measurement of reactive oxygen species (ROS) after stripe rust infection. Histochemical assays for superoxide anion radicals (${\mathrm{O}}_{\mathrm{2}}^{\mathrm{\cdot –}}$) and hydrogen peroxide (H₂O₂) by nitro blue tetrazolium (NBT) (A) and 3,3-diaminobenzidine (DAB) (B) staining, respectively. Then, ${\mathrm{O}}_{\mathrm{2}}^{\mathrm{\cdot –}}$ production (C) and the H₂O₂ content (D) were measured. Values are means ± SD from three independent biological replicates. Different letters depict significant differences between the susceptible (Sy95-71) and resistant (CN19) wheat cultivars (P < 0.05). CK, un-inoculated wheat plants.