Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 28;6:779. doi: 10.3389/fpls.2015.00779

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Chen, Cui, Su, Yuan, Yuan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Thylakoid protein phosphorylation after wheat stripe rust infection of the susceptible (Sy95-71) and resistant (CN19) wheat cultivars. Thylakoid proteins extracted from the inoculated and un-inoculated wheat plants were fractionated by SDS-PAGE in 12% acrylamide separation gel with 6 M urea. Immunoblot analysis of thylakoid membrane proteins was performed using anti-phosphothreonine antibodies (A). Loading was based on an equal amount of chlorophyll (1 μg chlorophyll). The SDS-PAGE results after Coomassie blue staining (CBS) are shown in the bottom panel (B). CK, un-inoculated wheat plants. (C) Quantification of immunoblot data. Results are presented relative to the amount of respective CK (100%). Asterisks indicates statistically significant differences at the P < 0.05 level. Values are means ± SD from three independent biological replicates.