Table 1.
Relevant E. coli engineered strains for SA production.
| Producing strain | Phenotypic traits | Special comments | Culture conditions | Title (g/L) | Yield (mol SA/mol glc) | Reference |
|---|---|---|---|---|---|---|
| SA112 | BW25113ΔaroKL, Ppps::PlacQ1,PcsrB::PlacQ1Pt5-pps,PT5-csrB, 5Ptac-tktA | CIChE evolved to optimize SA production | Shake flasks cultures with 10 g/L glc, 1 g/L peptone | 1.70 | 0.25 | Cui et al. (2014) |
| DHPYAAS-T7 | DH5α ΔptsHIcrr, ΔaroKL, ΔydiB pAOC-TGEFB:aroE, aroB, glk, tkta, aroFfbr | Plasmid overexpression of SA related genes | Shake flasks cultures (50 mL), M9 broth supplemented with 25 g/L glycerol, 10 g/L peptone, 15 g/L YE | 1.066 | 0.23 | Chen et al. (2012) |
| PB12.SA22 | JM101 ΔptsH, ptsI, crr::Kmr ΔarokL::cmr pJLBaroGfbrtktA, pTOPO-aroBaroE | Laboratory evolved PTS− glucose− into glucose+ derivative phenotype | 1 L batch bioreactor 25 g/L glc and 15 g/L YE | 7.05 | 0.22 | Escalante et al. (2010) |
| SA5 | B0013 ΔarokL::dif ΔptsG::dif ΔydiB:dif ΔackA-pta::dif pTH-aroGfbr-ppsA-tktA | Plasmid over expression of SA related genes | 7 L fed-batch bioreactor, initial 15 g/L glc supplemented with AA and vitamins | 14.6 | 0.3 | Chen et al. (2014) |
| SA114 | BW25113ΔaroKL, Ppps::PlacQ1, PcsrB::PlacQ1 PT5-pps,PT5-csrB, 5Ptac-tktA, 5Ptac-pntAB | CIChE evolved to optimize SA | Shake flasks, 10 g/L glc, 1 g/L peptone | 2.99 | 0.31 | Cui et al. (2014) |
| SP1.1pts-/pSC6.090B | DH5α ΔptsH-ptsI-crr ΔserA::aroB ΔaroL::Tn10 Δ aroK::Cmr Ptacglf glk, aroFfbttktA, PtacaroE, serA | Heterologous glk and glf from Z. mobilis to restore glc transport and phosphorylation in PTS− glc− phenotype | 10 L fed-batch bioreactor 55–170 mM Glc + 15 g/L YE | 84 | 0.33 | Chandran et al. (2003) |
| SA116 | BW25113ΔaroKL, Ppps::PlacQ1, PcsrB::PlacQ1 PT5-pps, PT5-csrB, 5Ptac-tktA, 5Ptac-nadK | CIChE evolved to optimize SA | Shake flasks 10 g/L glc, 1 g/L peptone | 3.12 | 0.33 | Cui et al. (2014) |
| AR36 | JM101 ΔptsH, ptsI, crr::Kmr ΔarokL::cmr ΔpykF ΔlacI pTrcAro6-aroB, tktA, aroGfbr, aroE, aroD zwf | Constitutive strong over expression by synthetic operon on plasmid | 1 L batch bioreactor 100 g/L glc + 15 g/L YE | 41.8 | 0.42 | Rodriguez et al. (2013) |
glc, glucose; YE, yeast extract.