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Substrate deformations as a function of substrate stiffness. (A) Substrate deformation field of one representative cell grown on a ~100 Pa substrate (cell outline in black). Deformation directions are indicated by white arrows, absolute deformations are shown by false colors. Scale bar: 10 μm. (B) Peak deformations reached a maximum on substrates of around G′ ~300–1000 Pa. N, number of analyzed cells. *p < 0.05; ***p < 0.001.
