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. 2015 Sep 24;6:1001. doi: 10.3389/fmicb.2015.01001

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Copyright © 2015 Wang, Kong, Zhang, Jiang, Zheng, Zang, Hao and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MRP-hFg interaction promotes the adherence of S. suis to hCMEC/D3 cells. (A) The adherent ratio of S. suis to hCMEC/D3 cells in the presence of hFg. S. suis 05ZYH33 and 05ZYH33Δmrp mutant were pretreated with a series of concentrations of hFg before infecting a hCMEC/D3 cell monolayer. The adherence ability of S. suis was evaluated by adherence assay. Values represent percent (mean ± S.D.) of total S. suis inoculum bound to the monolayers. (B) The adherent ability of S. suis to hCMEC/D3 cells evaluated by fluorescent microscopy. S. suis 05ZYH33 and 05ZYH33Δmrp mutant were labeled by BCECF (green) before infection, and hCMEC/D3 cell monolayers were treated or untreated with Fg (500 μg/ml). F-actin was strained with Rhodamine-labeled Phalloidin (red), nuclei were stained with DAPI (blue). *P < 0.05.