FIGURE 2.

MRP-hFg interaction increases the traversal ability of S. suis across the hCMEC/D3 cell monolayer. (A) The traversal rate of S. suis across the hCMEC/D3 monolayers. Confluent hCMEC/D3 cell monolayers pretreated with or without Fg were challenged with S. suis 05ZYH33 and 05ZYH33Δmrp for 25 min. The traversed bacteria in the basolateral chamber were enumerated by colony plate count. (B) The transendothelial permeability assay. Fg pretreated S. suis 05ZYH33 or 05ZYH33Δmrp were incubated with hCMEC/D3 cell monolayers in the presence of Lucifer yellow (200 μM) for 30 min. The amount of Lucifer yellow in the basolateral chamber was quantified with a spectrophotometer. D-mannitol was used as positive control as it disrupts cell–cell junctions. *P < 0.05, **P < 0.01.