. 2015 Sep 24;6:255. doi: 10.3389/fphys.2015.00255

Table 1.

Buffering and diffusion parameters.

Parameter	Description	Value
b $_{m}^{t o t}$	Total concentration of Troponin C (stationary buffer)	53.0 μM
b $_{s}^{t o t}$	Total concentration of Calmodulin (mobile buffer)	133.0 μM
B $_{s r}^{t o t}$	Total concentration of SR buffer	1500.0 μM
b $_{F l u o - 4}^{t o t}$	Total concentration of Fluo-4	133.0 μM
k $_{s}^{+}$	On rate for Troponin C binding	0.043 μM⁻¹ ms⁻¹
k $_{s}^{-}$	Off rate for Troponin C binding	0.026 ms⁻¹
k $_{m}^{+}$	On rate for Calmodulin binding	0.8 μM⁻¹ ms⁻¹
k $_{m}^{-}$	Off rate for Calmodulin binding	0.2 ms⁻¹
k $_{s r}^{+}$	On rate for SR buffer binding	0.1 μM⁻¹ ms⁻¹
k $_{s r}^{-}$	Off rate for SR buffer binding	60.0 ms⁻¹
k $_{F l u o - 4}^{+}$	On rate for Fluo-4 binding	0.0488 μM⁻¹ ms⁻¹
k $_{F l u o - 4}^{-}$	Off rate for Fluo-4 binding	0.0439 ms⁻¹
D $_{b}^{m}$	Diffusion constant of Troponin C	0.04 μm²/ms
D_B	Diffusion constant of SR buffer	0.01 μm²/ms
D	Diffusion constant of calcium	0.22 μm²/ms
D_S	Diffusion constant of SR calcium	0.20 μm²/ms
D_Fluo−4	Diffusion constant of Fluo-4	0.033 μm²/ms
τ_d	Refill-flux constant	0.5 ms
c₀	Starting [Ca²⁺]_i	0.275 μM