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. 2015 Sep 25;6:778. doi: 10.3389/fpls.2015.00778

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Copyright © 2015 Zeng, Liu, Pan, Hayward and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profile of miRNAs and their target genes in ripening fruits of Lycium barbarum L. The total RNAs were isolated from fruits at four developmental stages referring to S1 stage (green fruit, 3 days before color break), S2 stage (the color-break stage), S3 stage (light-color, 3 days after color break), and S4 stages (ripe fruit, 6 days after color break). These results were presented as mean ± SD of three biological replicates. The LbβFFase is predicted to be the target gene of miR164d, as well as LbGRAS for miR171a, Lbcid14 for miR5538, and LbOACPT for miR167a. The expression profile of miR164d, miR171a, miR167a, and miR5538 tested by qRT-PCR was identical to that of small RNA sequencing.