Table 2.
Expression level of novel miRNAs identified in Lycium barbarum L. ripening fruits.
| miRNA | miRNA Sequence (5′–3′) | S1 | S2 | S3 | S4 | miRNA* Sequence (5′–3′) | miRNA* | LcmiRNA |
|---|---|---|---|---|---|---|---|---|
| miR01 | UUGCCGACUCCACCCAUACCAC | 21591.9 | 31871.2 | 63553.1 | 7079.7 | GGGGUGGGUGGGGCGGUA | Yes | |
| miR02 | AAACCCUCAGCGAUCCAUAAC | 5548.5 | 3433.7 | 2436.3 | 206.0 | UUGUGGGUUGCAGGGGGUUUU | Yes | LC35 |
| miR03a | UAGGGCGUUCGGAUCCUUCUGC | 1808.1 | 3278.3 | 4055.3 | 827.0 | AGAAGGAUGCUGACGCCCUUGC | Yes | LC1 |
| miR04 | UUACCUGAUCCUGGCAUACCAA | 464.1 | 3235.9 | 5440.3 | 62.0 | GGCGUGUCAAUAUUGGGUAAGA | Yes | |
| miR05b | AAUCCUUCUGCAAUCCAUAAC | 2927.0 | 2183.2 | 2543.6 | 236.3 | UGUGGGUUGCAGAGGGUUUUA | Yes | LC11 |
| miR06 | UCUUACCAAUACCUCCCAUUCC | 546.8 | 1932.3 | 3784.2 | 426.8 | AGUGGGAGGUGUUGUAAGAUA | Yes | LC16 |
| miR07c | UAGAAAGAGUUUGUAGGCGAG | 390.6 | 1759.2 | 901.2 | 208.2 | UGCCUACAAACUCUUUCUAUA | Yes | LC14 |
| miR08 | GAUCAUGUGGUAGCUUCACC | 94.2 | 2381.0 | 1377.1 | 22.9 | UGAAGCUGCCAGCAUGAUCUAAAC | Yes | LC4 |
| miR09 | UUGCCAAUUCCCCCCAUUCCGA | 9906.8 | 14928.8 | 26649.3 | 4115.8 | GGAGUGGGUGGCAUGGCAAGA | Yes | LC02 |
| miR10 | UUAGAUUCACGCACAAACUUG | 204.5 | 904.3 | 419.4 | 25.1 | CGUUUGUGCCUGGAUCUGACA | Yes | LC9 |
| miR11 | UCCAAUCUCCUCGCCCAUAUUU | 576.7 | 717.1 | 600.8 | 89.3 | UAUGGAGAGGUGAUUGGAGA | Yes | LC17 |
| miR12 | CAUCACAGGUUACUCCAUCCCA | 195.3 | 522.8 | 842.7 | 59.8 | GGAUGGACUGAAAUGUGAUAAG | Yes | |
| miR13 | GCGAAAGUCGUCUGUGACCCG | 11542.7 | 4776.1 | 1851.1 | 437.9 | UGUCGCAGGUGACUUUCGCCC | Yes | |
| miR14 | CCCCAUGGACGACCUAAAUACG | 500.9 | 310.9 | 132.6 | 11.1 | UAUUUAGGUCGACAAGGGGUA | Yes | |
| miR15 | AAUCCCGGGAUUGUAGUGUUAUUU | 335.4 | 381.5 | 259.4 | 18.5 | CGAUAUAACUAAUCCUGGGAU | Yes | LC33 |
| miR16b | AAGCCUUCCGCAAUCCAUAAC | 255.0 | 222.6 | 156.0 | 11.8 | UGUGGGUUGUAGAGGGUUUUA | Yes | |
| miR17c | UUUUCCCUUGAAUACUCACUU | 80.4 | 137.8 | 156.0 | 6.6 | UGAGUAUUCAAGGGAAAAUGG | Yes | |
| miR18 | CGCACCCAACAUGGCUCCAUC | 0.0 | 31.8 | 148.2 | 3.0 | UGGUGCCACGCUGUGUGCGAC | Yes | |
| miR19 | AAUGCUGAAAGAGUCGUGCCU | 89.6 | 45.9 | 9.8 | 1.5 | CACGACUCUUUCAGCAUUUUU | Yes | |
| miR20 | AAAACCCCCGCGAAUUGCAACUUU | 50.5 | 49.5 | 21.5 | 0.7 | AGUUGCAAUUCCCGGGAUUUUAGC | Yes | |
| miR21 | CAAUAACAAACUCCAGGAGUG | 0.0 | 28.3 | 15.6 | 0.0 | CUCCUGUAGUAUUGUUAAUUGG | Yes | |
| miR22 | CCCUUUGAACUGAGAUGUGCCU | 57.4 | 21.2 | 7.8 | 2.2 | CACUCUCAGUUCACAGAGGCGG | Yes | |
| miR23 | UUUUUGGAGAGUUCGAGCAAC | 34.5 | 17.7 | 11.7 | 0.0 | UGCUCGGACUCUUCAAAAAAU | Yes | |
| miR24 | GAAUAGGAAAAACAACUU | 0.0 | 53.0 | 25.4 | 0.0 | CAUUGGUUUCCUGGGCUGUCA | Yes | |
| miR25 | CAAAGGCCACAAGAUUCACUU | 0.0 | 0.0 | 15.6 | 0.0 | GCAAAUGUUGUGGUCUUAGCA | Yes | LC31 |
| miR26 | AAGACAGGGGUAUAUUUGAAAACU | 6.9 | 24.7 | 25.4 | 0.7 | CAAAUAUACCCGUCUUGGCGA | Yes | |
| miR27 | AUAAUACUUGGAAUAUGCCCU | 363.0 | 823.1 | 470.1 | 14.0 | CAUAUUCCAAGUAUUAUGGGA | Yes | LC32 |
| miR28 | UCGAACCCGUGACCUCAAGCC | 18.4 | 98.9 | 119.0 | 40.6 | CUUGAGGUUGCGGGUUCGAUA | Yes | |
| miR29 | AUAAACAGACCCUCAAACUUGGCC | 18.4 | 45.9 | 56.6 | 0.0 | AAGUUUGAGGGCUUGUUUAU | Yes | |
| miR30 | UUAAAAAGGGUUAUGUAGUGGC | 50.5 | 1271.7 | 524.7 | 6.6 | CACUACAAAGCCCUUCUUAUUU | No | LC3 |
| miR31 | UGAUACUGCACUUGGAUACCU | 608.8 | 3465.5 | 2016.9 | 344.8 | GUAUCCAAGUGCAGUAUCAUA | Yes | |
| miR32 | AUGUAGGAUACUCAGCACUCGCGG | 23.0 | 7.1 | 9.8 | 11.8 | GGAGGUUGAGUAUCACGCAGUA | No | |
| miR33 | AGCGCCUCCGGGUCCUGUCGACUA | 36.8 | 45.9 | 37.1 | 43.6 | ACUGAUCCGGUAGGCUGCGCUGCA | Yes | |
| miR34 | UUAUAUGGACUUGGACAAUCC | 66.6 | 21.2 | 21.5 | 0.7 | AUUGCUCAAGUCCAUUUAAGA | No | |
| miR35 | AAGAGAGUUUCGCUGGGCUUUAGC | 11.5 | 42.4 | 27.3 | 3.7 | UUCGGCCCAGUAAUUUUUUUCA | No | |
| miR36 | CGAAAUUCGUGCGUGAAAGGACCA | 6.9 | 53.0 | 19.5 | 1.5 | GCCUUUCACGCAUGAAAUUCGUG | No | |
| miR37 | GCUCACUGCUCUAUCUGUCACC | 2.3 | 21.2 | 31.2 | 2.2 | UGACAGAAGAGAGUGAGCAC | No | |
| miR38 | CAUAGGAUUCUUGGGCAUGCU | 3855.2 | 2246.7 | 1410.3 | 2677.4 | AUGUUCAAGAAUCCUAUGCUA | Yes | LC25 |
| miR39 | AGGCAUGAGUAUGCCGGGUCCGGC | 48.2 | 38.9 | 11.7 | 0.0 | CGGACUCAGCAUAUUUAUGCAACG | No | |
| miR40 | UCUUUCCUACGCCUCCCAUACC | 15076.2 | 17638.3 | 66518.0 | 8712.3 | UGUGGGUGGGGUGGAAAGAUU | Yes | |
| miR41 | ACUUGGGACGAGUUCGUGACUGCU | 27.6 | 0.0 | 19.5 | 22.2 | CAGACAUGGAUAUUGCCCGACUAC | No | |
| miR42 | UUGCUCGGACUCUUUAAAAAU | 4.6 | 7.1 | 5.9 | 0.7 | AUUUUUGGAGAGUCCGAGCAAUAU | No | |
| miR43 | AAUGAACCGGACUCCCUUAAG | 9.2 | 0.0 | 0.0 | 3.0 | UAAGCGAGUCUGGCCCAUUGU | No | |
| miR44 | AGAGGCCUGUAGACAUGUAUG | 2.3 | 14.1 | 0.0 | 0.7 | GCCAGUCUAGGGUGCCGUUCUGC | Yes | |
| miR45a | UAUGGCGUUCGGAUCCUUCUAC | 73.5 | 60.1 | 93.6 | 19.9 | GGAUAUCGACGCCCUUGCUCUUA | Yes | |
| miR46 | ACUCCCUCCGUUCACUUUUACUUG | 29.9 | 24.7 | 25.4 | 19.2 | UAAAAGUGAACGGAGGGAG | Yes | |
| miR47 | CAAAUCCCUCUGCGAUCCAUA | 2692.7 | 4016.6 | 6109.3 | 277.6 | UGGGUCGCUGAAGGAUUGAUG | Yes | |
| miR48 | UUUUCUACCUGAACUAUCACC | 193.0 | 35.3 | 13.7 | 1.5 | AUAGUUCAGGUAGAAAAAGGG | No | |
| miR49 | UUAUCUGCCCCUGCCUUUGCAUCC | 6.9 | 10.6 | 11.7 | 0.0 | AGUAGUUGUAGGUGGUGGAUUAGU | Yes | |
| miR50 | CAAACAAGUUGGGGCCAGCU | 75.8 | 63.6 | 29.3 | 3.0 | AGCCGACCUCAACUAGUUUAG | Yes |
Expression level of novel miRNAs were presented in TPM value (Transcripts Per Million). miRNAs marked by “a,” “b,” or “c” are processed from the same transcripts. LcmiRNAs column indicated that these novel miRNAs in Lycium chinense were also detected in L. barbarum.