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. 2015 Sep 25;9:126. doi: 10.3389/fnana.2015.00126

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Copyright © 2015 Adori, Barde, Bogdanovic, Uhlén, Reinscheid, Kovacs and Hökfelt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the distribution of NPS mRNA-positive with NPS-immunoreactive neurons in the rat brainstem 1—the periventricular cluster. (A) Schematic drawing from the atlas by Paxinos and Watson (2007), indicating distribution of NPS-positive cell bodies (arrows and red dots) around the fourth ventricle. All schematic drawings are presented in higher magnification in the Supplementary Material. (B) shows distribution of the transcript and (C) of the peptide immunoreactivity at approximately the same level. Note the similar distribution of the NPS neurons in the periventricular cluster visualized with in situ hybridization (NPS mRNA) and immunohistochemistry (NPS peptide). The section in (C) is from a colchichine-treated rat. (A) is reproduced from Paxinos and Watson (2007), with permission. Scale bar: 200 μm (C), applies to (B,C).