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. 2015 Sep 25;5:68. doi: 10.3389/fcimb.2015.00068

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Copyright © 2015 Lucas, Ouellette, Kabeiseman, Cichos and Rucks.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron micrograph analysis of the effect of syntaxin 10 siRNA knockdown on chlamydial development. Syntaxin 10 (Stx10) or non-targeting (NT) siRNA-treated HeLa cells were infected with C. trachomatis serovar L2 for 36 h and then processed for transmission electron microscopy as described in Materials and Methods. For these studies, 40 images each from either Stx10 or NT-siRNA treated cells from 2 independent experiments were examined. Representative images are shown in (A) and Supplemental Figure 1. These images were used to quantify chlamydial developmental forms (B), total numbers of organisms per inclusion (C), or inclusion diameter (D). All values were graphed to display the mean and standard error of the mean using GraphPad Prism 6 software. Statistical analysis included ordinary One-Way ANOVA with Tukey's multiple comparison test (B) and Student's t-test (C,D).