Table 4.
Predicted PSMs of S. capitis strain AYP1020 were detected in the culture supernatant using HPLC followed by mass spectrometry.
| PSM | Predicted amino acid sequencea | Molecular Weightb | m/z of peaks (+ H+)c | Retention time (min) |
|---|---|---|---|---|
| α | fMSDIINQIVKVVKGLIEKFTNK | 2547.1 | 1274.2 (+2) | 21.3 |
| 849.8 (+3) | ||||
| 637.4 (+4) | ||||
| β1a | fMQKLAEAIANTVKAGQDHDWTKLGTSIVDIVENGVSALTKVFGG | 4643.3 | 1548.2 (+3) | 20.5 |
| 1161.4 (+4) | ||||
| 929.7 (+5) | ||||
| β1c | fMTKLAEAIANAVKAGQDQDWAKLGTSIVGIAENGISLLGKVFGF | 4563.3 | 1521.9 (+3) | 23.5 |
| 1141.6 (+4) | ||||
| 913.4 (+5) | ||||
| β1d | fMQKLAEAIANTVKAGQDHDWAKLGTSIVGIAENGIGLLGKVFGF | 4599.3 | 1150.3 (+4) | 19.5 |
| 920.3 (+5) | ||||
| β2 | MEKLFDAIRNTVDAGINQDWTKLGTSIVDIVDNGVKVISKFIGA | 4822.5 | 1608.7 (+3) | 25.2 |
| 1206.5 (+4) | ||||
| δ | MGIVTTIIEIVKTIVDLVKKFKK | 2617.3 | 872.5 (+3) | 22.5 |
| 654.6 (+4) | ||||
| fMGIVTTIIEIVKTIVDLVKKFKK | 2646.3 | 1323.7 (+2) | 27 | |
| 882.9 (+3) | ||||
| ε | MFIVNLIKKAIEFFKGLFGNKK | 2586.2 | 862.7 (+3) | 17.5 |
| δ-toxin | MAADIVSTISDFIKWIIDTIKKFKK | 2912.5 | 971.6 (+3) | 19.5 |
| 729.2 (+4) | ||||
| fMAADIVSTISDFIKWIIDTIKKFKK | 2941.5 | 1471.2 (+2) | 25.2 | |
| 981.3 (+3) |
a
Formylation of the amino-terminus is indicated as f.
b
The molecular weight is calculated based on predicted amino acid sequence.
c
The numbers of protons added to the peptides are suggested.