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. 2015 Sep 24;9:365. doi: 10.3389/fncel.2015.00365

Figure 2.

Figure 2

Age-related changes in the ultrastructure of the neurogenic niches. (A,A′) Astrocytes accumulate dense bodies (box) in their cytoplasm during aging. Scale bar: 2 micra. (B,B′) Detail of intermediate filaments (arrows) in astrocytic cells. Note that they are more abundant in aged cells. Scale bar: 500 nm. (C,C′) Detail of lipid droplets in ependymal cells, displaying a larger size during aging. Scale bar: 5 micra. (D,D′) Ependymal cells are flattened in the aged brain, resulting in large gaps between ciliary tufts (arrows). Scale bar: 2 micra. (E) Under scanning electron microscopy, whole-mount preparation of the lateral ventricle shows a deep network of axons (arrows) in the aged brain. Scale bar: 5 micra. (F) DAPI (4′,6-diamidino-2-phenylindole) fluorescent staining shows a remarkable RMS (arrows) from the lateral ventricle to the OB in the young brain. Scale bar: 1 mm. (G) Conversely, the RMS is not evident in the aged brain. Scale bar: 1 mm. b, astrocyte; e, ependymal cell; Cb, cerebellum; Ctx, cerebral cortex; Lp, lipid droplets; Lv, lateral ventricle; OB, olfactory bulb. Images (F,G) have been adapted with permission from Capilla-Gonzalez et al. (2013).