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. 2015 Sep 24;6:1023. doi: 10.3389/fmicb.2015.01023

FIGURE 1.

FIGURE 1

The tspo gene (Pfl01_2811) of Pseudomonas fluorescens Pf0-1 forms an operonic structure with Pfl01_2810 that is expressed transiently in the bacterial growth course. (A) Schematic representation of the genomic environment of tspo and of transcriptional fusions pTSPO and pHK-TSPO. Gray bars represent the beginning of the luxCDABE reporter cassette of the promoterless pAB133 vector. The position of the studied promoter region is indicated relatively to the translational initiation start of each ORF. (B) Co-transcription of Pfl01_2810 and tspo by reverse transcription-PCR (RT-PCR) assay. RT-PCR was assayed on tspo (1), Pfl01_2810 (2) and on the putative operonic structure Pfl01_2810-tspo (3), using primers located into tspo (1) or Pfl01_2810 (2) ORFs, or into both tspo and Pfl01_2810 ORFs (3). RT-PCR achieved on total RNA did not lead to a PCR fragment (line 4, negative control). L: Ladder 1kb+ (Biorad®), primers sequences are given in Table 2. (C) Growth curves in microtitre wells in Luria-Bertani (LB) medium at 28°C and relative luminescence (RL) activity of pTSPO and pHK-TSPO in P. fluorescens Pf0-1. (D) Growth curves in erlenmeyer in LB medium at 28°C and RL activity of pHK-TSPO in P. fluorescens Pf0-1.