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. 2015 Sep 24;6:1023. doi: 10.3389/fmicb.2015.01023

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Copyright © 2015 Leneveu-Jenvrin, Bouffartigues, Maillot, Cornelis, Feuilloley, Connil and Chevalier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A putative RpoH-consensus binding site is located in Pfl01_2810-tspo promoter region: effect of temperature on growth and TSPO expression. (A) Sequence alignment of the putative RpoH binding sites identified by MEME in the regions located upstream of dnaK in E. coli and groEL, dnaK, grpE, and Pfl01_2810 in P. fluorescens Pf0-1, relatively to the E. coli RpoH consensus sequence. Localization of this sequence is given relatively to the +1 translational start site. (B) Growth and transcriptional activity of P. fluorescens Pf0-1 containing pHK-TSPO reporter fusion in microtiter plates cultured at 28 or 32°C. (C) Relative expression of Pfl01_2810 when bacteria were grown at 32°C compared to 28°C by qRT-PCR assays. Significances of differences between mean values were assessed using the Student’s t-test with significance set as ^∗P < 0.05.