Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| Pseudomonas fluorescens | ||
| Pf0-1 | Wild type | Silby et al., 2009 |
| P. aeruginosa | ||
| PAO1 | Wild type | Woodruff and Hancock, 1989 |
| PAOU | PAO1ΔalgU | Bazire et al., 2010 |
| rpoH | Transposon mutant rpoH::IS;phoA/hah-Tcr | University of Washington mutant library (Jacobs et al., 2003) |
| Plasmids | ||
| pAB133 | Gmr; pBBR1MCS-5-based cloning vector containing the promoterless luxCDABE operon. | Bazire et al., 2005 |
| pTSPO | Gmr; pAB133-based plasmid with the 253-bp tspo upstream fragment at the SacI and SpeI sites. | This study |
| pHK-TSPO | Gmr; pAB133-based plasmid with the 227-bp Pfl01_2810-tspo upstream fragment at the SacI and SpeI sites | This study |
| pHK-TSPO-88 | Gmr; pAB133-based plasmid with the 89-bp Pfl01_2810-tspo upstream fragment at the SacI and SpeI sites. | This study |
| pHK-TSPO-157 | Gmr; pAB133-based plasmid with the 70-bp Pfl01_2810-tspo upstream fragment at the SacI and SpeI sites | This study |
| pHK-TSPO-227 | Gmr; pAB133-based plasmid with the 69-bp Pfl01_2810-tspo upstream fragment at the SacI and SpeI sites | This study |