Table 2.

Primers used in this study.

Primer	Name	Sequence (5′-3′)∗
HKF		GCCCTGCTCAACCTGTGTAT
HKR		CCTAGCGGTTTGGTGGTAAA
TSPOF		AACAAGCCGAAATTCACACC
TSPOR		CACAGCAGGACGAGAATGAG
HKTSPOF		CGGGTCAGTGAATTGATGTG
HKTSPOR		GGTGTGAATTTCGGCTTGTT
F1	pTSPO	taataagagctcAGCGCATGAGCATGATGTA
R1	pTSPO	taataaactagtCCGACAGGTGCAGTCAAT
F2	pHK-TSPO	taataagagctcATGAACGGTCGACAACTGG
R2	pHK-TSPO	taataaactagtTTCTTACTCCTTGGCCGC
F3	pHK-TSPO-227	taataagagctcAGCGCATGAGCATGATGTAC
R3	pHK-TSPO-227	taataaactagtCGTTGCCAGAGTGCCTGT
F4	pHK-TSPO-157	taataagagctcCGGCCAGGCATTGTTGG
R4	pHK-TSPO-157	taataaactagtGTCGTATCGGGCTAGCACT
F5	pHK-TSPO-88	taataagagctcCTTGCTCCATCAGTGAAACTTAA
R5	pHK-TSPO-88	taataaactagtACAGGTGCAGTCAATATCGG
16SF		CTGGTAGTCCACGCCGTAAAC
16SR		CCAGGCGGTCAACTTAATGC
Pfl012810F		AGCTGATGCCGCTCTACGA
Pfl012810R		GTACGG-TCGCGGAGAATTTTC

^∗All primers used in this study were synthesized by Eurogentec and are based on P. fluorescens Pf0-1 genome sequence (http://www.pseudomonas.com). Nucleotides in lowercase are not complementary to the target sequence. Underlined nucleotides indicate restriction endonuclease sites inserted within primer sequences.