Table 2.
Primers used in this study.
| Primer | Name | Sequence (5′-3′)∗ |
|---|---|---|
| HKF | GCCCTGCTCAACCTGTGTAT | |
| HKR | CCTAGCGGTTTGGTGGTAAA | |
| TSPOF | AACAAGCCGAAATTCACACC | |
| TSPOR | CACAGCAGGACGAGAATGAG | |
| HKTSPOF | CGGGTCAGTGAATTGATGTG | |
| HKTSPOR | GGTGTGAATTTCGGCTTGTT | |
| F1 | pTSPO | taataagagctcAGCGCATGAGCATGATGTA |
| R1 | pTSPO | taataaactagtCCGACAGGTGCAGTCAAT |
| F2 | pHK-TSPO | taataagagctcATGAACGGTCGACAACTGG |
| R2 | pHK-TSPO | taataaactagtTTCTTACTCCTTGGCCGC |
| F3 | pHK-TSPO-227 | taataagagctcAGCGCATGAGCATGATGTAC |
| R3 | pHK-TSPO-227 | taataaactagtCGTTGCCAGAGTGCCTGT |
| F4 | pHK-TSPO-157 | taataagagctcCGGCCAGGCATTGTTGG |
| R4 | pHK-TSPO-157 | taataaactagtGTCGTATCGGGCTAGCACT |
| F5 | pHK-TSPO-88 | taataagagctcCTTGCTCCATCAGTGAAACTTAA |
| R5 | pHK-TSPO-88 | taataaactagtACAGGTGCAGTCAATATCGG |
| 16SF | CTGGTAGTCCACGCCGTAAAC | |
| 16SR | CCAGGCGGTCAACTTAATGC | |
| Pfl012810F | AGCTGATGCCGCTCTACGA | |
| Pfl012810R | GTACGG-TCGCGGAGAATTTTC |
∗All primers used in this study were synthesized by Eurogentec and are based on P. fluorescens Pf0-1 genome sequence (http://www.pseudomonas.com). Nucleotides in lowercase are not complementary to the target sequence. Underlined nucleotides indicate restriction endonuclease sites inserted within primer sequences.