Table 6.

Summary of heterologous expression of proteins of thermophilic and alkaliphilic actinobacteria.

Actinobacteria	Enzymes	Expressi on Host	Optimum pH and temperature	Km	Vmax	References
Acidothermus cellulolyticus 11B	Thermostable endoxylanase (Xyn10A)	E. coli BL21	pH 6 and 90°C	0.53 mg/ml	ND	Barabote et al., 2010
Acidothermus cellulolyticus	Endoglucanase (E1)	Pichia pastoris	pH 5.1 and 80°C	372 ± 50 μM	0.523 ± 0.070 μM/min	Lindenmuth and McDonald, 2011
Acidothermus cellulolyticus	Glucose isomerase	E. coli BL21	pH 6.5 and 80°C	0.40 M	6.41	Mu et al., 2012
Acidothermus cellulolyticus	Alditol oxidase	E. coli BL21	pH 6–9	Varies with different substrates	Varies with different substrates	Winter et al., 2012
Saccharomonospora viridis	Xylanase (Svixyn10A)	E. coli BL21	8.0 and 60°C	0.68 mg/ml	217.93 U/mg	Wang et al., 2012
Nocardiopsis prasina OPC-131	Chitinase	E. coli BL21	pH 9.0	ND	ND	Tsujibo et al., 2003
Isoptericola jiangsuensis CLG	Chitinases IS-chiA and IS-chiB	E. coli BL21	pH 5 and 30°C and pH 9 and 50°C, respectively	11.66 and 17 μM, respectively	10.93 and 12.24 μmol min−1 mg−1, respectively	Wu et al., 2011a
Brachystreptospora xinjiangensis OM-6 and Nocardiopsis alba OK-5	Proteases	E. coli BL21	pH 10	ND	ND	Gohel and Singh, 2012b
Thermobifida fusca	Cytochrome P450 monooxygenase CYP154H1	E. coli BL21	50°C	ND	ND	Schallmey et al., 2011
Thermobifida fusca TM51	β-D-mannosidase	E. coli BL21	7.17 and 53°C	180 μM	5.96 μmol min−1 mg−1,	Beki et al., 2003
Thermobifida halotolerans	Endoglucanase	E. coli BL21	pH 8 and 55°C	12.02 mg/ml	105.26 μM min−1	Zhang et al., 2011
Thermobifida fusca	Trehalose synthase	Pichia pastoris	25°C and pH 6.5	ND	ND	Wei et al., 2004
Corynebacterium glutamicum	Flavin containing monooxygenase	E. coli BL21	pH 8 and 25°C	Varies with substrate	Varies with substrate	Ameria et al., 2015

^*ND, not determined.