Table 6.
Actinobacteria | Enzymes | Expressi on Host | Optimum pH and temperature | Km | Vmax | References |
---|---|---|---|---|---|---|
Acidothermus cellulolyticus 11B | Thermostable endoxylanase (Xyn10A) | E. coli BL21 | pH 6 and 90°C | 0.53 mg/ml | ND | Barabote et al., 2010 |
Acidothermus cellulolyticus | Endoglucanase (E1) | Pichia pastoris | pH 5.1 and 80°C | 372 ± 50 μM | 0.523 ± 0.070 μM/min | Lindenmuth and McDonald, 2011 |
Acidothermus cellulolyticus | Glucose isomerase | E. coli BL21 | pH 6.5 and 80°C | 0.40 M | 6.41 | Mu et al., 2012 |
Acidothermus cellulolyticus | Alditol oxidase | E. coli BL21 | pH 6–9 | Varies with different substrates | Varies with different substrates | Winter et al., 2012 |
Saccharomonospora viridis | Xylanase (Svixyn10A) | E. coli BL21 | 8.0 and 60°C | 0.68 mg/ml | 217.93 U/mg | Wang et al., 2012 |
Nocardiopsis prasina OPC-131 | Chitinase | E. coli BL21 | pH 9.0 | ND | ND | Tsujibo et al., 2003 |
Isoptericola jiangsuensis CLG | Chitinases IS-chiA and IS-chiB | E. coli BL21 | pH 5 and 30°C and pH 9 and 50°C, respectively | 11.66 and 17 μM, respectively | 10.93 and 12.24 μmol min−1 mg−1, respectively | Wu et al., 2011a |
Brachystreptospora xinjiangensis OM-6 and Nocardiopsis alba OK-5 | Proteases | E. coli BL21 | pH 10 | ND | ND | Gohel and Singh, 2012b |
Thermobifida fusca | Cytochrome P450 monooxygenase CYP154H1 | E. coli BL21 | 50°C | ND | ND | Schallmey et al., 2011 |
Thermobifida fusca TM51 | β-D-mannosidase | E. coli BL21 | 7.17 and 53°C | 180 μM | 5.96 μmol min−1 mg−1, | Beki et al., 2003 |
Thermobifida halotolerans | Endoglucanase | E. coli BL21 | pH 8 and 55°C | 12.02 mg/ml | 105.26 μM min−1 | Zhang et al., 2011 |
Thermobifida fusca | Trehalose synthase | Pichia pastoris | 25°C and pH 6.5 | ND | ND | Wei et al., 2004 |
Corynebacterium glutamicum | Flavin containing monooxygenase | E. coli BL21 | pH 8 and 25°C | Varies with substrate | Varies with substrate | Ameria et al., 2015 |
*ND, not determined.