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. 2015 Sep 17;6:755. doi: 10.3389/fpls.2015.00755

FIGURE 2.

FIGURE 2

The subcellular localization and transcriptional activation analysis of pepper CaNAC2. (A) The subcellular localization of pepper CaNAC2 in onion epidermal cells. The fused pBI221-GFP-CaNAC2 and pBI221-GFP constructs were introduced into onion epidermal cells by biolistic bombardment. The GFP signals were observed under confocal microscope; (B) Transcriptional activation analysis of CaNAC2 in yeast strain AH109. CaNAC2 represents the fusion protein of the GAL4 DNA-binding domain and CaNAC2; pGBKT7 was used as control. The culture solution of the transformed yeast was streaked on SD/-Leu/-Trp medium and SD/-Ade/-His/-Leu/-Trp medium. The plates were incubated for 3 days.