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. 2015 Sep 28;6:786. doi: 10.3389/fpls.2015.00786

FIGURE 3.

FIGURE 3

Transcripts of defense-related genes in tomato leaves in response to mycorrhizal colonization and pathogen infection. The tomates were pre-inoculated with mycorrhizal fungus Funneliformis mosseae and later inoculated with A. solani, the causal agent of early blight disease of tomato. Quantitative real time RT-PCR was used to detect the transcripts of six defense-related genes encoding PAL (A), LOX (B), allene oxide cyclase (AOC) (C), pathogen-related proteins (PR1) (D), β-1,3-glucanase (basic type PR-2) (E), and chitinase (PR-3) (F). Four treatments included: (1) CK: control plants without pathogen and mycorrhizal inoculation; (2) As: plants inoculated with A. solani only; (3) Fm: plants inoculated with F. mosseae only; (4) Fm+As: plants inoculated with both F. mosseae and A. solani. Values are means ± SE from three sets of independent experiments with three pots per treatment for each set of experiments. Significant differences (P < 0.05 using Tukey post hoc test) among treatments in a group are indicated by different letters above bars.