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. 2015 Sep 28;6:786. doi: 10.3389/fpls.2015.00786

FIGURE 4.

FIGURE 4

Levels of defense-related enzymes in leaves of tomatoes with mycorrhizal colonization and pathogen infection. Wild-type (WT) and mutant plants (35S::PS and spr2) of tomato were pre-inoculated with mycorrhizal fungus Funneliformis mosseae and later inoculated with A. solani, the causal agent of early blight disease of tomato. Four defense-related enzymes are β-1,3-glucanase (A), LOX (B), PAL (C), and chitinase (D). Enzymatic activities were analyzed 18 (a), 65 (b), 100 (c) and 140 h (d) after pathogen inoculation. Four treatments included: (1) CK: control plants without pathogen and mycorrhizal inoculation; (2) As: plants inoculated with A. solani only; (3) Fm: plants inoculated with F. mosseae only; (4) Fm+As: plants inoculated with both F. mosseae and A. solani. Three tomato genotypes included: (1) WT: wild type plant; (2) 35S::PS: Prosystemin-overexpressing 35S::PS plant; (3) spr2: JA biosynthesis mutant plant. Values are means ± SE from three sets of independent experiments with three pots per treatment for each set of experiments. Significant differences among treatments were tested at P = 0.05 by Tukey post hoc test.