Table 1.
Specific primer for real-time PCR.
| Gene | Accession No. | Primer sequence (5′ to 3′) | PCR product size |
|---|---|---|---|
| LeLOX | U13681 | F: 5′-ATCTCCCAAGTGAAACACCACA-3′ R: 5′-TCATAAACCCTGTCCCATTCTTC-3′ |
109 bp |
| LeAOC | AW624058 | F: 5′-CTCGGAGATCTTGTCCCCTTT-3′ R: 5′-CTCCTTTCTTCTCTTCTTCGTGCT-3′ |
115 bp |
| LePR1 | DQ159948 | F: 5′-GCCAAGCTATAACTACGCTACCAAC-3′ R: 5′-GCAAGAAATGAACCACCATCC-3′ |
139 bp |
| LePR2 | M80604 | F: 5′-GGACACCCTTCCGCTACTCTT-3′ R: 5′-TGTTCCTGCCCCTCCTTTC-3′ |
81 bp |
| LePR3 | Z15140 | F: 5′-AACTATGGGCCATGTGGAAGA-3′ R: 5′-GGCTTTGGGGATTGAGGAG-3′ |
128 bp |
| LePIN2 | X94946 | F:5′-AATTATCCATCATGGCTGTTCAC-3′ R: 5′- CCTTTTTGGATCAGATTCTCCTT-3′ | 254 bp |
| LePAL | AW035278 | F: 5′-CTGGGGAAGCTTTTCAGAATC-3′ R:5′-TGCTGCAAGTTACAAATCCAGAG-3′ |
150 bp |
| LeUBI3 | X58253 | F: 5′- TCCATCTCGTGCTCCGTCT -3′ R:5′-GAACCTTTCCAGTGTCATCAACC-3′ |
144 bp |