Figure 5.

Competition experiment. Competition experiment in LB medium (A–D) and during colonization of the mouse large intestine (E–H) for MG1655 and MG1655 ΔDARS1 (A,E, respectively), MG1655 and MG1655 ΔDARS2 (B,F, respectively), MG1655 and MG1655 ΔDARS1 ΔDARS2 (C,G, respectively), and MG1655 and MG1655 ΔdatA (D,H, respectively). At the indicated times, samples were homogenized, diluted, and plated as described in Materials and Methods. Bars represent the standard error of the log10 mean number of CFU per mL (competition experiment in LB medium) or CFU per gram of feces (mice experiment).