Table 2.
Bacterial OTUs obtained from clone library analyses, number of clones in each OTU, closest BLASTn match (identity percentage in parenthesis) and source, class and lowest taxonomic classification (confidence percentages in parenthesis).
| OTU | Clones | T-RF abundance HaeIII/MspI (±SE) | BLASTn (acc. no., % match) | Phylum or class | Lowest taxon |
|---|---|---|---|---|---|
| 1 | 8a | 14.8/15.5 (±1.4/±1.7) | Sponge-associated (EU236387, 99) | Alphaproteobacteria (100) | G. Hoeflea (96) |
| 2 | 3a | n.d./28.4d (n.d./±1.9) | Sediment (GQ246350, 99.4) | Alphaproteobacteria (100) | G. Rhodobium (42) |
| 3 | 2b | n.d./0.92 (n.d./±0.2) | Environmental (GQ274271, 98.1) | Gammaproteobacteria (100) | G. Spongiibacter (31) |
| 4 | 2b | n.d./28.4d (n.d./±1.9) | Sediment (FM242451, 97.3) | Alphaproteobacteria (100) | G. Sneathiella (100) |
| 5 | 1c | 7.7/28.4d (±1.0/±1.9) | Environmental (HM591412, 99) | Alphaproteobacteria (100) | G. Kordiimonas (100) |
| 6 | 1b | 16.2/1.9 (±1.0/±1.2) | Environmental (EU491139, 93.4) | Gammaproteobacteria (100) | G. Ectothiorhodosinus (27) |
| 7 | 1b | 3.3/5.0 (±0.3/±0.8) | Coral-associated (GU119659, 97.4) | Cyanobacteria (97) | F. Chlorarachniophyceae (36) |
| 8 | 1b | n.d./n.d. (n.d./n.d.) | Sponge-associated (FJ269336, 99.4) | Acidobacteria (99) | O. Gp21 (99) |
| 9 | 1b | 33.7/30.0 (±1.9/±1.9) | Sponge-associated (AM259846, 95.4) | Gammaproteobacteria (100) | G. Ectothiorhodosinus (42) |
| 10 | 1b | n.d./n.d. (n.d./n.d.) | Sponge-associated (JX280383, 100) | Deltaproteobacteria (84) | G. Desulfocurvus (35) |
G., genus; F., family; and O., order. aPresent in both clone libraries. bPresent in DF2 library. cPresent in DF7 library. dOTUs 2, 4, and 5 contribute to the same TRF (MspI digest).