Table 4.

Relative levels of phenolic compounds in WT and Ljgln2-2 leaves under control conditions (normal watering and suppressed PR).

Compound	Ljgln 2-2/WT
kaempferol-3,7-di-O-rhamnosidea, b	0.82±0.28
kaempferol-3-O-glucosyl-7-O-rhamnosidea, b	0.65±0.20*
kaempferol-3-O-galactosyl-7-O-rhamnosideb	0.70±0.23*
kaempferol-3-O-glucosyl (1-2)-galactoside-7-O-rhamnosidea, b	0.69±0.23*
kaempferol-3-O-glucosyl (1-2)-glucoside-7-O-rhamnosidea, b	1.10±0.62
kaempferol-6-deoxyhexoseb	0.56±0.33*
Total kaempferol (after acid hydrolysis)	0.97±0.34
Quercetin-6-deoxyhexose, -hexoseb	2.46±1.53*
Quercetin-6-deoxyhexose, -hexoseb	1.57±1.10
Quercetin-6-deoxyhexose, 6-deoxyhexoseb	1.44±0.98
total quercetine (after acid hydrolysis)	1.87±0.91*
flavonol-6-deoxyhexoseb	0.60±0.25*
p-coumaric acid	0.64±0.41
p-ferulic acid	0.94±0.51
Simple phenylpropanoid	0.76±0.48
vestitol	1.12±0.34
Total tannins	1.05±0.15

The differences in the relative levels of phenolic compounds between WT and Ljgln2-2 mutant plants are reported as the ratio between metabolite levels in Ljgln2-2 and the levels of the same metabolite in WT plants. Total crude extracts from leaves were obtained in 50% methanol and analyzed by HPLC as described in materials and methods, with the exception of vestitol, that due to its low polarity was quantified in leaf extracts obtained in 100% methanol. The different compounds were identified in HPLC chromatograms employing DAD detector. Tannins were determined spectrophotometrically. Identities of compounds were verified by

^aNMR or

^bLC/ESI-MS.

For the compounds in italics it was not possible to determine their accurate chemical structure (e.g., for the two different quercetin-6-deoxyhexose,-hexoses isomers, that showed different retention times). Significant difference between levels in mutant and WT at

^*p < 0.05 (n = 4) according to Student's t-test.

Data are the mean ± S.D. of six independent biological replicates.