Figure 2. Inflammatory and immune response transcriptional programs activated after traumatic muscle injury.

(a) Gene expression profiles of pro- and anti-inflammatory genes (IL-1b & Socs3 and Il-6 & Arid5a), which show similar activation profiles and are part of networks with opposing function, (red – injured samples, blue – uninjured samples, IL-1b – squares & solid line, Socs3 – circles & dashed line, IL-6 – squares & solid line, Arid5a – circles & dashed line). Arid5a operates to reduce IL-6 stability, indicating the inflammatory response to injury is transcriptionally regulated on multiple levels. (b) Heatmaps of significantly up-regulated (red) or down-regulated (blue) genes for different functional categories. (c) Example of alternative splicing detected during early time period. Il1rl1 (ST2) undergoes an increase expression in the ST2L isoform (blue), which has previously been shown to promote proliferation and activation of anti-inflammatory macrophages.