Figure 4. Muscle tissue microenvironment signaling after traumatic muscle injury.

(a) The left side illustrates the RNA-Seq read coverage for the fibronectin (Fn1) gene for the EDA exon during different time points after the injury. The MISO + (percent spliced in) values are on the right and show a shift in the EDA exon for the middle time points, indicating an increased detection of the ED-A splice variant. Detection of the splice variant decreases back to control values at the 672 h time point. (b) Top—Gene expression profile of Myogenin (MyoG), a transcription factor that regulates terminal differentiation of the myogenic program, and Id2, a helix-loop-helix protein that inhibits myogenic factor activity and modulates the terminal myogenic differentiation program. Bottom—Gene expression profile of Myomaker (Tmem8c), a transmembrane protein that fuses adjacent myoblasts, which remained upregulated until 672 h after the injury.