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. 2015 Sep 18;5:14090. doi: 10.1038/srep14090

Figure 7. AGE-induced VE-cadherin activation requires Src.

Figure 7

(a) Pretreatment of PP2 prevented AGE-induced VE-cadherin phosphorylation. ECs were pretreated with PP2 (15 μmol/L) for 90 min before exposed to 100 μg/mL AGEs for 1 h. (b) Pretreatment of Src siRNA prevented AGE-induced VE-cadherin phosphorylation. ECs were transfected with Src siRNA or control siRNA for 48 h before exposed to 100 μg/mL AGEs for 1 h. (c,d) Effects of K298M and Y530F on AGE-induced VE-cadherin phosphorylation. ECs were transfected with K298M or Y530F for 48 h before exposed to 100 μg/mL AGEs for 1 h. VE-cadherin and its phosphorylation form were determined by western blotting. The ratio of immunointensity between the phosphorylation of VE-cadherin (p-VE658) and β-actin were calculated. n = 3, *P < 0.05 versus control, #P < 0.05 versus AGEs or Mock + AGEs. (e) Down-regulation of Src prevented AGE-induced adherence junction dissociation. HUVECs were pretreated with PP2 (15 μmol/L) for 90 min or transfected with Src siRNA for 48 h, followed by incubation with or without 100 μg/mL AGEs for 8 h. Immunostaining for VE-cadherin (green) and DAPI (blue) were shown.