Figure 3.

Nova1 and Ncoa3 are required for the repressive activity of a subset of neuronal miRNAs

• A–F Luciferase reporter gene assays performed in rat cortical neurons (14 DIV) (rat hippocampal neurons in (D)) that had been transfected at 11 or 12 DIV with the indicated 3′UTRs fused to the luc gene of pGL4 together with the given shRNA expression construct. In (A, B), additional shRNA-resistant (^R) expression constructs for Nova1 and Ncoa3, respectively, or the empty expression vector (GFP) were co-transfected. Relative luciferase activity (RLA) represents the ratio of firefly reporter activity to Renilla control reporter activity normalized to a basal condition without shRNA expression vector. Values are the mean ± standard deviation from at least three independent experiments. One-way ANOVA: P < 0.0001 (A, C, E), P < 0.005 (B, D, F). Tukey’s HSD (A–D) and unpaired t-test (E, F): *P < 0.05, **P < 0.01.