Skip to main content
. 2015 Jun 23;34(17):2237–2254. doi: 10.15252/embj.201490643

Figure 9.

Figure 9

The regulation of Ago2 is required for Ncoa3-dependent miRNA activity and dendritogenesis

  • A Quantification of Ago2 overexpression in hippocampal neurons (12 DIV) that have been transfected with GFP, the indicated shRNA expression vectors, and either empty Flag/HA (control vector) or Flag/HA-Ago2 after six DIV by anti-Ago2 immunocytochemistry. Measured was the average signal intensity in the neuronal soma (exemplary images in Supplementary Fig S8C), and values presented are the mean ± standard deviation from three independent experiments (in each 12 cells per condition). *P < 0.05 (pairwise t-test).
  • B Reporter gene assay performed in rat hippocampal neurons (14 DIV) that had been transfected with pGL4-LIN41 WT 3′UTR together with the indicated shRNA vectors and either empty Flag/HA (control vector) or Flag/HA-Ago2 three days before. Relative luciferase activity (RLA) represents the ratio of firefly reporter to the internal Renilla control reporter activity normalized to basal condition without additional expression vector. Values are the mean ± standard deviation from three independent experiments. One-way ANOVA: P < 0.0003; Tukey’s HSD test: **P < 0.01.
  • C Representative traces of neurons (12 DIV) that have been transfected as described in (A). Scale bar = 50 μm.
  • D, E Results from Sholl analysis performed as in Fig6 with neurons treated as in (A). One-way ANOVA: P = 0.0005; Tukey’s HSD test: **P < 0.01.