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. 2014 Jun 13;25(10):3290–3302. doi: 10.1093/cercor/bhu125

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© The Author 2014. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Areal variations in numbers of Tbr2 IP derived neurons with reciprocal differences between Layer 4 and 5 dorsomedial and ventrolateral cortices of the presumptive visual cortex. A The Tbr2 IPC derived Ai9+ cells are distributed across all cortical layers throughout the cortex. We compared their variation in number in selected areas labeled with boxes A′ and A″ in A (CCtx: Caudal Cortex). A′ and A″ We counted the number of Ai9+ cells in each layer between the dorsomedial (A′) and ventrolateral regions (A″). B Similar proportion of Ai9+ cells could be seen in Layers 2/3 (58.33 ± 4.6 vs. 46.67 ± 4.5, n = 2 brains, P = 0.12 multiple t-test with correction) but there was an appreciable difference in Layer 4 (66.67 ± 5.2 vs. 46.51 ± 3.9, n = 2, P = 0.048) and in Layer 5 (12.50 ± 3.5 vs. 38.24 ± 2.7, n = 2 brains, P = 0.014). No difference was noticed when all layers were grouped together suggesting against differences in cell densities (35.1 vs. 35.62). Scale bar 100 μm.