Figure 1.

(A, left panels) Immunoblots from retinal explants cultured under hypoxia/reoxygenation for 16 or 24 hours. Black arrowheads show intact protein bands, and white arrowheads show their breakdown products. N, normoxia; H, hypoxia; H+SNJ, hypoxia plus the calpain inhibitor SNJ-1945; H&R, hypoxia for 16 hours followed by reoxygenation for 8 hours (hypoxia/reoxygenation); H&R+SNJ, hypoxia/reoxygenation plus SNJ-1945. (B, right panels) Immunoblots of normal, noncultured retinal soluble proteins incubated in the test tube for 30 minutes without Ca²⁺ (N), with Ca²⁺ (Ca), or with Ca²⁺ plus SNJ-1945 (Ca+SNJ). Endogenous GAPDH was used as the normalizing loading control.